MICaB Graduate Program
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Janet L. Schottel, Ph.D.
Department of Biochemistry, Molecular Biology and Biophysics
Washington University, 1977, Ph.D.
Microbial-based Cancer Therapies; Regulation of Gene Expression; Stress Responses
One of my primary research interests is the development of microbial-based therapies for cancer. An attenuated strain of Salmonella enterica Typhimurium is currently used in these studies. This organism is a gram-negative facultative bacterium that can invade and divide with macrophages and other cell types and thrives in hypoxic areas of tumors. Previous reports have indicated that administration of this organism significantly reduced tumor size and number in mouse models of metastatic osteosarcoma, primary neuroblastoma and liver adenocarcinoma. Current work is focused on optimizing the cancer suppressing activity of this bacterium by expressing various genes that modulate the immune system and determining the most effective protocol for administration of this organism to the mice with potential future applications to humans.
Another research interest focuses on the regulation of gene expression in cells in response to stresses such as desiccation, nutrient deprivation, culture density, osmotic stress, heat shock, and mechanical stress. Our approaches include studying the regulation of transcription, mRNA degradation and protein synthesis in cells grown under a variety of culture conditions.
Selected Recent Publications:
- Drees, J.J., M.J. Mertensotto, L.B. Augustin, J.L. Schottel, A.S. Leonard and D.A. Saltzman. 2013. Soluble production of a biologically active single-chain antibody against murine PD-L1 in Escherichia coli. Manuscript in review.
- Walker, J.D., D.C. Brooks, K. Hammond, B.A. Fall, R.W. Peifer, R. Schnell, and J.L. Schottel. 2013. Practice makes perfect? Assessing the effectiveness of online practice exams in blended learning biology classes. In A. Picciano, C. Dziuban & C. Graham, eds., Research Perspectives in Blended Learning. New York: Routledge
- Scherber, C., J.L. Schottel and A. Aksan. 2009. Membrane phase behavior of Escherichia coli during desiccation, rehydration, and growth recovery. BBA-Biomembranes. 1788:2427-2435.
- Schottel, J.L., Orwin, .M., Anderson, C.R. and Flickinger, M.C. 2008. Spatial expression of a mercury-inducible green fluorescent protein within a nanoporus latex-based biosensor coating. J. Ind. Microbiol. Biotechnol. 35(4):283-90.
- Flickinger, M.C., Schottel, J.L., Bond, D.R., Aksan, A. and Scriven, L.E. 2007. Painting and printing lliving bacteria: engineering nanoporus biocatalytic coatings to preserve microbial viability and intensify reactivity. Biotechnol. Prog. 23(1):2-17.
- Schottel, J., C. Anderson and M. Flickinger. 2005. Mercury biosensors: Spatial expression of mercury-induced green fluorescent protein or luciferase in Escherichia coli immobilized in latex biocatalytic coatings. Cryobiology 51:384.
- Ryan, A.D., L.L. Kinkel, and J.L. Schottel. 2004. Effect of Pathogen Isolate, Potato Cultivar, and Antagonist Strain on Potato Scab Severity and Biological Control. Biocontrol Science and Technology, 14:301-311.
- Flickinger, M.C., S. Charaniya, C. Solheid, C.R. Anderson, O.K. Lyngberg, H. Ge, J.L. Schottel, and L.E. Scriven. 2004. Catalytic Coatings - Multi-Layer, Permeable, Reactive Latex Coatings Containing Living Bacteria: Applications for Biosensors, Bio-Electronic Devices, and Biocatalysis. European Coating Conference, Smart Coatings III; Berlin, Germany.